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Anti-CFC1 antibody

Anti-CFC1 antibody
别名: CFC1
适用物种: Human  
验证应用: WB,IHC-P  
种属: 兔多抗  
储存条件: -20℃ 
Anti-CFC1 antibody
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY6906-18R-50 μl 兔多抗 现货 1500.00 1500
ZY6906-18R-100 μl 兔多抗 现货 2500.00 2500
熔点:
密度:
储存条件: -20℃

 

Anti-CFC1 antibody

 

  • 概述

  • 产品描述NODAL coreceptor involved in the correct establishment of the left-right axis. May play a role in mesoderm and/or neural patterning during gastrulation.

  • 产品名称Anti-CFC1 antibody

  • 分子量15 KDa

  • 种属反应性Human

  • 验证应用WB,IHC-P

  • 抗体类型兔多抗

  • 免疫原KLH conjugated synthetic peptide derived from human CFC1 1-100/223

  • 偶联Non-conjugated

  • 性能

  • 形态Liquid

  • 浓度1 mg/mL.

  • 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

  • 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • 亚型IgG

  • 纯化方式affinity purified by Protein A

  • 亚细胞定位Cell membrane. Secreted. Does not exhibit a typical GPI-signal sequence. The C-ter hydrophilic extension of the GPI-signal sequence reduces the efficiency of processing and could lead to the production of an secreted unprocessed form. This extension is fo

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  • ​其它名称CFC 1
    CFC1
    CFC1_HUMAN
    CFC1B
    CR 1
    Cripto 1
    Cripto
    Cripto FRL 1 cryptic family 1
    CRYPTIC
    Cryptic family 1
    Cryptic family protein 1
    Cryptic gene
    Cryptic protein
    DTGA2
    FLJ77897
    FRL 1
    HTX2
    MGC133213.

    more
  • 应用

    WB:1:500-2000
    IHC-P:1:400-800

  •  

    Fig1: Protein: Hela(human) lysate at 40ug;
    Primary: rabbit Anti-CFC1 at 1:300;
    Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
    Predicted band size: 15 kD
    Observed band size: 15 kD

     

    Fig2: Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CFC1) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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