Anti-HER4 / ErbB4 antibody [4G19]

Anti-HER4 / ErbB4 antibody [4G19]

• 概述

• 产品描述The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. The gene encoding ErbB-4 is expressed as a full length protein, which produces a short membrane-anchored cytoplasmic domain fragment and a long ectodomain fragment. The short fragment is heavily tyrosine phosphorylated and possesses tyrosine kinase catalytic activity toward an exogenous substrate. Proteolytic cleavage of ErbB-4 is promoted by the binding of heregulin. ErbB-4 is involved in cell proliferation and differentiation and its expression is highest in breast carcinoma cell lines, normal skeletal muscle, heart, pituitary, brain and cerebellum.
• 产品名称Anti-HER4 / ErbB4 antibody [4G19]
• 分子量180kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IP,IF,IHC-P
• 抗体类型小鼠单抗
• 免疫原Amino acids 1280-1308 at C-terminus of ErbB-4 of human origin.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cell membrane, Nucleus, Mitochondrion
• 其它名称
  • 4ICD antibody
  • ALS19 antibody
  • Avian erythroblastic leukemia viral oncogene homolog 4 antibody
  more

• 应用

  WB: 1:100-1:1,000
  IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
  IF: 1:50-500
  IHC-P: 1:50-500
  FC: 1 μg per 1 x 106 cells

•  

  

  Fig1: Western blot analysis of ErbB-4 expression in control (A) and ErbB-4 transfected (B) NIH/3T3 whole cell lysates.

   

  

  Fig2: Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and ErbB-4 transfected (solid orange histogram) NIH/3T3 cells. Dotted pink histogram represents the isotype control, normal mouse IgG2a.

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！