Anti-AUP1 antibody [1H6A1]

Anti-AUP1 antibody [1H6A1]

• 概述

• 产品描述The protein encoded this gene is involved in several pathways including quality control of misfolded proteins in the endoplasmic reticulum and lipid droplet accumulation. Lipid droplets are organelles in the cytoplasm that store neutral lipids such as cholesterol esters and trigylycerides to prevent the overabundance of free cholesterol and fatty acids in cells, but also to act as storage for other metabolic processes, such as membrane biogenesis. Reduced expression of this gene results in reduced lipid droplet clustering, a function that is dependent on ubiquitination of the protein. This protein contains multiple domains including a hydrophobic N-terminal domain, an acetyltranferase domain, a ubiquitin-binding CUE domain, and a UBE2B2-binding domain (G2BR). Alternative splicing results in multiple transcript variants.
• 产品名称Anti-AUP1 antibody [1H6A1]
• 分子量53kDa
• 种属反应性Human,Mouse
• 验证应用WB
• 抗体类型小鼠单抗
• 免疫原Purified recombinant fragment of human AUP1 (AA: 229-410) expressed in E. Coli.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS with 0.05% sodium azide.
• 亚型IgG2b
• 纯化方式Protein G purified.
• 亚细胞定位Endoplasmic reticulum membrane.
• 其它名称
  • AA589454 antibody
  • Ancient ubiquitous protein 1 antibody
  • AUP 1 antibody
  mor

• 应用

  WB: 1:500-1:2,000

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  Fig1: Western blot analysis of AUP1 against human AUP1 (AA: 229-410) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig2: Western blot analysis of EM1711-17 against HEK293 (1) and AUP1 (AA: 229-410)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig3: Western blot analysis of against A431 (1), NIH/3T3 (2), Hela (3), SW480 (4), CHO3D10 (5), A549 (6), and SPC-A-1 (7) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

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