Anti-SIRT3 antibody [2A-1B]

Anti-SIRT3 antibody [2A-1B]

• 概述

• 产品描述The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent mating-type loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologues, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT3 is a NAD-dependent deacetylase that contains one deacetylase sirtuin-type domain. The SIRT3 protein is widely expressed and localizes to the mitochondira where it is processed by mitochondrial processing peptidase (MPP) to yield a final product. This processing is most-likely necessary for its enzymatic activity.
• 产品名称Anti-SIRT3 antibody [2A-1B]
• 分子量44 kDa
• 种属反应性Human
• 验证应用WB,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Mitochondrion matrix.
• 其它名称
  • hSIRT 3 antibody
  • hSIRT3 antibody
  • Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody
  more

• 应用

  WB: 1:500-1:1,000
  FC: 1:50-1:200

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  Fig1: Western blot analysis of SIRT3 on human SIRT3 recombinant protein using anti-SIRT3 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of SIRT3 on HEK293 (1) and SIRT3-hIgGFc transfected HEK293 (2) cell lysate using anti-SIRT3 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of SIRT3 on PANC-1 cell lysate using anti-SIRT3 antibody at 1/1,000 dilution.

   

  

  Fig4: Flow cytometric analysis of Hela cells with SIRT3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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