Anti-WIF1 antibody [E7-G5]

Anti-WIF1 antibody [E7-G5]

• 概述

• 产品描述The Wnt genes are a group of conserved, Cysteine-rich, secreted glycoproteins that are required for numerous developmental processes including embryogenesis, asymmetric cell division and central nervous system (CNS) patterning. Wnt association with the transmembrane spanning receptor frizzled activates dishevelled, which downregulates glycogen synthase kinase (GSK) through serine phosphorylation. Reduced levels of active GSK causes accumulation of b-catenin and subsequent regulation of developmentally significant Wnt target genes. Wnt antagonists such as dickkopf (Dkk), frizzled-related protein (sFRP) and Wnt inhibitory factor-1 (WIF-1), are necessary to ensure normal spatial and temporal patterns of Wnt activity during developmental processes. WIF-1 is a 379-amino acid secreted protein that contains an N-terminal signal sequence, a 150-amino acid WIF domain, five epidermal growth factor-like repeats and a 45-amino acid C-terminal hydrophilic domain.
• 产品名称Anti-WIF1 antibody [E7-G5]
• 分子量42 kDa
• 种属反应性Human,Mouse
• 验证应用WB,ICC,IHC-P
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Secreted.
• 其它名称
  • WIF-1 antibody
  • wif1 antibody
  • WIF1_HUMAN antibody
  more

• 应用

  WB: 1:500
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200

•  

  

  Fig1: Western blot analysis of WIF1 on different cell lysates using anti-WIF1 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: Hela
  Lane 2: NIH/3T3
  Lane 3: NTERA-2

   

  

  Fig2: ICC staining WIF1 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human ovary tumour tissue using anti-WIF1 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-WIF1 antibody. Counter stained with hematoxylin.

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