Anti-ARHGDIA antibody [G8-C3]

Anti-ARHGDIA antibody [G8-C3] 

• 概述

• 产品描述Members of the Ras superfamily of small GTP-binding proteins are critical mediators of diverse cell signaling pathways, including those leading to cell proliferation, cytoskeletal organization and secretion. The counter-conversion of the active GTP-bound form of these proteins to their inactive GDP-bound form is influenced by two types of regulatory proteins: those that alter the intrinsic GTPase activity of the GTP-binding proteins and those that alter the rate of GDP/GTP exchange. Guanine nucleotide-releasing factors (GRFs) increase the GDP dissociation rate, while GDP-dissociation inhibitors (GDIs) decrease the dissociation rate. Rho GDIα, also known as ARHGDIA or GDIA1, is a 204 amino acid member of the Rho GDI family of proteins. Localized to the cytoplasm, Rho GDIα inhibits the dissociation of GDP from Rho proteins, thereby preventing GTP from binding to and subsequently activating Rho proteins. In humans, Rho GDIα can be phosphorylated at Ser 101 by p21-activated kinase (αPAK), an event that inhibits Rho GDIα activity and may result in positive feedback regulation of certain Rho GDIα target proteins.
• 产品名称Anti-ARHGDIA antibody [G8-C3]
• 分子量23kDa
• 种属反应性Human,Mouse,Monkey
• 验证应用WB,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm..
• 其它名称
  • ARHGDIA antibody
  • fa96g11 antibody
  • GDIA 1 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:100-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of ARHGDIA on human ARHGDIA recombinant protein using anti-ARHGDIA antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of ARHGDIA on different cell lysates using anti-ARHGDIA antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: Jurkat
  Lane 2: HeLa
  Lane 3: NIH-3T3
  Lane 4: C6
  Lane 5: K562
  Lane 6: COS7

   

  

  Fig3: ICC staining ARHGDIA (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Flow cytometric analysis of Hela cells with ARHGDIA antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！