Anti-LGALS1 antibody [C5-H11]

Anti-LGALS1 antibody [C5-H11]

• 概述

• 产品描述Galectins are a family of soluble b-galactoside-binding animal lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. Specifically, Galectin-1 is an autocrine regulator of cell-proliferation that plays a role in the maintenance of G0 and in the control of G2 traverse. Galectin-1, also known as LGALS1, is the protein product of a single gene linked to human chromosome 22q13.1. The Galectin-1 protein contains 135 amino acids, a single internal EcoRI site and a polyadenylation signal. Galectin-1 can localize to both intracellular and extracellular space. Galectin-1 is expressed in human placenta, human lung, HL-6, HepG2 and CEM cells.
• 产品名称Anti-LGALS1 antibody [C5-H11]
• 分子量15 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Secreted. Extracellular space. Extracellular matrix. Nucleus
• 其它名称
  • 14 kDa laminin-binding protein antibody
  • 14 kDa lectin antibody
  • Beta galactoside binding lectin antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of Galectin 1 on human Galectin 1 recombinant protein using anti- Galectin 1 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of Galectin 1 on HEK293 (1) and Galectin 1-hIgGFc transfected HEK293 (2) cell lysate using anti- Galectin 1 antibody at 1/1,000 dilution.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti- Galectin 1 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti- Galectin 1 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of Hela cells with Galectin 1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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