Anti-PSMB8 antibody [H2-A5]

Anti-PSMB8 antibody [H2-A5]

• 概述

• 产品描述The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.
• 产品名称Anti-PSMB8 antibody [H2-A5]
• 分子量22 kDa
• 种属反应性Human,Rat
• 验证应用WB,ICC,IHC-P
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm. Nucleus.
• 其它名称
  • ALDD antibody
  • D6S216 antibody
  • D6S216E antibodyLarge multifunctional peptidase 7 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:100-1:500
  IHC-P: 1:100-1:500

•  

  

  Fig1: Western blot analysis of PSMB8 on human PSMB8 recombinant protein using anti-PSMB8 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of PSMB8 on different cell lysates using anti-PSMB8 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: Hela
  Lane 2: MCF-7
  Lane 3: A431
  Lane 4: RAJI
  Lane 5: MOTL4
  Lane 6: PC-12

   

  

  Fig3: ICC staining PSMB8 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded intima cancer tissue using anti-PSMB8 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PSMB8 antibody. Counter stained with hematoxylin.

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