Anti-SNAI2 antibody [D5-B6]
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概述
- 产品描述The SNAIL family of developmental regulatory proteins is a group of widely conserved zinc-finger proteins that regulate transcription and include the mammalian proteins SLUG, SNAI 1 (the human homolog of Drosophila SNAIL) and Smuc. SNAI 1 and SLUG are expressed in placenta and in adult heart, liver, and skeletal muscle. SNAI 1, and the corresponding mouse homolog Sna, each contain three classic zinc fingers and one atypical zinc finger, while SLUG contains five zinc finger regions and a transcriptional repression domain at the amino terminus, which enables SLUG to act as a negative regulator of gene expression. SLUG is implicated in the generation and migration of neural crest cells in human embryos and also contributes to limb bud development. In addition, SLUG also constitutes a cellular anti-apoptotic transcription factor that effectively prevents apoptosis in murine pro-B cells deprived of IL-3. The SNAIL-related gene from murine skeletal muscle cells, Smuc, is highly expressed in skeletal muscle and thymus and can, likewise, repress gene transcription. Smuc preferentially associates with CAGGTG and CACCTG E-box motifs (CANNTG) on DNA and involves the five putative DNA-binding zinc finger domains at the C-terminal region of Smuc.
- 产品名称Anti-SNAI2 antibody [D5-B6]
- 分子量30 kDa
- 种属反应性Human
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型小鼠单抗
- 免疫原Recombinant protein
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度2 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG1
- 纯化方式Protein A purified.
- 亚细胞定位Nucleus,cytoplsm
- 其它名称
- MGC10182 antibody
- Neural crest transcription factor Slug antibody
- Protein snail homolog 2 antibody
more
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应用
WB: 1:500-1:2000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of SNAI2 on human SNAI2 recombinant protein using anti-SNAI2 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of SNAI2 on HEK293 (1) and SNAI2-hIgGFc transfected HEK293 (2) cell lysate using anti-SNAI2 antibody at 1/1,000 dilution.
Fig3: Western blot analysis of SNAI2 on MCF-7 cell lysate using anti-SNAI2 antibody at 1/1,000 dilution.
Fig4: ICC staining SNAI2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using anti-SNAI2 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using anti-SNAI2 antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of MCF-7 cells with SNAI2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
![Anti-SNAI2 antibody [D5-B6]](images/202012/goods_img/92749_G_1607941708157.jpg)
