Anti-Ephrin-A1 antibody
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概述
- 产品描述Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis.
- 产品名称Anti-Ephrin-A1 antibody
- 分子量24 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原The antiserum was produced against synthesized peptide derived from human EFNA1. AA range:66-115
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/ml
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 亚型IgG
- 纯化方式The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 亚细胞定位Cell membrane, Secreted.
- 其它名称
- B61 antibody
- ECKLG antibody
- EFL 1 antibody
more
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应用
WB: 1:500-1:2,000
IHC-P: 1:50-1:200
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Fig1: Western blot analysis of Ephrin-A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibodywas used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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