Anti-DDX39B antibody [B2-A2]

Anti-DDX39B antibody [B2-A2]

• 概述

• 产品描述BAT1 (spliceosome RNA helicase BAT1), also known as DEAD-box protein UAP56 (56 kDa U2AF65-associated protein), HLA-B associated transcript-1 or ATP-dependent RNA helicase p47, is a member of the DECD subfamily of DEAD-box helicases. Important for mRNA splicing and nuclear export, BAT1 interacts with the mRNA export factor ALY and also functions as a splicing factor, mediating the first ATP-dependent step of spliceosome assembly. BAT1 associates with transcription elongation factor THO proteins, forming the TREX complex, and also interacts with splicing machinery to form the exon junction complex. Due to alternative splicing events, BAT1 exists in two isoforms. DDX39 (DEAD box protein 39), like BAT1, is a member of the DEAD-box family of helicases. Localized to the nucleus and expressed in lung, brain, kidney, splenn, thymus and salivary gland, DDX39 functions in a similar manner to BAT1 and is involved in pre-mRNA splicing and mRNA export out of the nucleus. DDX39 expression is upregulated in lung squamous cell carcinoma, suggesting a role for DDX39 in tumorigenesis.
• 产品名称Anti-DDX39B antibody [B2-A2]
• 分子量49 kDa
• 种属反应性Human,Mouse
• 验证应用WB,ICC,IHC-P
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus. Nucleus speckle
• 其它名称
  • 0610030D10Rik antibody
  • 4F2-LC6 antibody
  • 56 kDa U2AF65-associated protein antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200

•  

  

  Fig1: Western blot analysis of DDX39B on human DDX39B recombinant protein using anti- UAP56 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of DDX39B on HEK293 (1) and DDX39B-hIgGFc transfected HEK293 (2) cell lysate using anti-DDX39B antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of DDX39B on different cells lysates using anti-DDX39B antibody at 1/1,000 dilution.
  Positive control: Line1: HEK293 Line2: Jurkat Line3: MCF-7 Line4: A431 Line5: NIH/3T3 Line6: Jurkat Line7: K562 Line8: HepG2

   

  

  Fig4: ICC staining DDX39B (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using anti-DDX39B antibody. Counter stained with hematoxylin.

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