Anti-Human IgM antibody
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概述
- 产品描述Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen. IgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The membrane-bound form is found in the majority of normal B-cells alongside with IgD. Membrane-bound IgM induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. It may cause death of cells by apoptosis. It is also found in soluble form, which represents about 30% of the total serum immunoglobulins where it is found almost exclusively as a homopentamer. After the antigen binds to the B-cell receptor, the secreted form is secreted in large amounts.
- 产品名称Anti-Human IgM antibody
- 分子量70/30 kDa
- 种属反应性Human
- 验证应用WB, IHC-P
- 抗体类型兔多抗
- 免疫原Native protein.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/ml.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein A purified.
- 亚细胞定位Cell Membrane and Secreted.
- 其它名称
- Immunoglobin heavy chain constant region mu edit item name - Immunoglobin heavy chain mu constant region antibody
- Immunoglobin heavy chain constant region mu antibody
- AGM1 antibody
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应用
WB: 1:500-1:2,000
IHC-P: 1:50-1:200
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Fig1: Western blot analysis of human IgM on Human plasma (1) and Raji (2) lysate using anti-Human IgM antibody at 1/1,000 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Human IgM antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Human IgM antibody. Counter stained with hematoxylin.
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