Anti-Hsp90 alpha antibody

Anti-Hsp90 alpha antibody

• 概述

• 产品描述The heat shock response was first described for Drosophila salivary gland cells and morphologically consists of a change in their polytene chromosome puffing patterns that involves de novo synthesis of a few proteins. Similar heat shock proteins were later discovered in bacterial chicken and mammalian cells, and have been subsequently studied in other organisms. A series of proteins including HSP 90, HSP 70, HSP 20-30 and ubiquitin are induced by insults such as temperature shock, chemicals and other environmental stress. A major function of HSP 90 and other HSPs is to act as molecular chaperones. HSP 90 forms a complex with glucocorticoid receptor (GR), rendering the non ligand-bound receptor transcriptionally inactive. HSP 90 binds the GR as a heterocomplex composed of either HSP 56 or Cyclophilin D, forming an aporeceptor comiplex. HSP 90 also exists as a dimer with other proteins such as p60/sti1 and p23, forming an apo-receptor complex with estrogen and androgen receptors.
• 产品名称Anti-Hsp90 alpha antibody
• 分子量90kDa

• 种属反应性Human,Mouse,Rat

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原peptide

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified

• 亚细胞定位Cytoplasm, Cell membrane, Melanosome

   

• 其它名称

  more

  • EL52 antibody

  • epididymis luminal secretory protein 52 antibody

  • Heat shock 86 kDa antibody

• 应用

  WB: 1:1,000-1:5,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis on different cell lysates using anti-HSP90 alpha rabbit polyclonal antibody. Positive control:
  Lane 1: Hela
  Lane 2: 293T
  Lane 3: PC-12
  Lane 4: NIH/3T3

   

  

  Fig2: Immunocytochemical staining of HepG2 cells using anti-HSP90 alpha rabbit polyclonal antibody.

   

  

  Fig3: Immunocytochemical staining of C2C12 cells using anti-HSP90 alpha rabbit polyclonal antibody.

   

  

  Fig4: Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-HSP90 alpha rabbit polyclonal antibody.

   

  

  Fig5: Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-HSP90 alpha rabbit polyclonal antibody.

   

  

  Fig6: Immunohistochemical analysis of paraffin- embedded mouse kidney tissue using anti-HSP90 alpha rabbit polyclonal antibody.

   

  

  Fig7: Flow cytometric analysis of Hela cells with HSP90 alpha antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

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