Anti-PIASy antibody
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概述
- 产品描述domain:The LXXLL motif is a coregulator signature that is essential for transcriptional corepression. Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Promotes PARK7 sumoylation. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. pathway:Protein modification; protein sumoylation. PTM:Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activitation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate. Belongs to the PIAS family. Contains 1 SAP domain. Contains 1 SP-RING-type zinc finger. subcellular location:Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies. subunit:Interacts with AR, GATA2, LEF1, TP53 and STAT1 (IFNG-induced). Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs) (By similarity). Interacts with TICAM1. Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G(1) phase. tissue specificity:Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.
- 产品名称Anti-PIASy antibody
- 分子量56 kDa
- 种属反应性Human,Mouse
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原The antiserum was produced against synthesized peptide derived from human PIAS4. AA range:451-500
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified.
- 其它名称
- PIAS4 antibody; PIASG antibody; E3 SUMO-protein ligase PIAS4 antibody; PIASy antibody; Protein inhibitor of activated STAT protein 4 antibody; Protein inhibitor of activated STAT protein gamma antibody; PIAS-gamma antibody;
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应用
WB: 1:500-1:2000
IHC-P: 1:100-1:300
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
