Anti-CD28 antibody

Anti-CD28  antibody

• 概述

• 产品描述Possibly involved in T-cell activation. CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins. When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen-presenting cells (APCs). The CD86 expression on antigen-presenting cells is constitutive (expression is independent of environmental factors). CD28 is the only B7 receptor constitutively expressed on naive T cells. Association of the TCR of a naive T cell with MHC:antigen complex without CD28:B7 interaction results in a T cell that is anergic. The drug TGN1412, which was produced by the German biotech company TeGenero, and unexpectedly caused multiple organ failure in trials, is a superagonist of CD28. Unfortunately, it is often ignored that the same receptors also exist on cells other than lymphocytes. CD28 has also been found to stimulate eosinophil granulocytes where its ligation with anti-CD28 leads to the release of IL-2, IL4, IL-13 and IFN-γ.

• 产品名称Anti-CD28 antibody

• 分子量25 KDa

• 种属反应性Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep

• 验证应用WB, IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human CD28 19-120/220

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式Affinity purified by Protein A

• 亚细胞定位Membrane; Single-pass type I membrane protein.

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• ​其它名称CD 28
  CD28 antigen
  CD28 molecule
  MGC138290
  T cell antigen CD28
  T cell specific surface glycoprotein
  T cell specific surface glycoprotein CD28
  Tp44
  CD28_HUMAN.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

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  Fig1: Sample:
  Spleen (Mouse) Lysate at 40 ug
  Primary: Anti-CD28 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 23 kD
  Observed band size: 23 kD

   

  

  Fig2: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum, C-0005) at 37℃ for 20 min;
  Incubation: Anti-CD28 Polyclonal Antibody, Unconjugated  1:200, overnight at 4℃, followed by conjugation to the secondary antibody (SP-0023) and DAB (C-0010) staining

   

  

  Fig3: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-CD28 Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody (SP-0023) and DAB (C-0010) staining

   

  

  Fig4: Tissue/cell: human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-CD28 Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody (SP-0023) and DAB (C-0010) staining

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