Anti-hnRNP DL antibody

Anti-hnRNP DL antibody

• 概述

• 产品描述Acts as a transcriptional regulator. Promotes transcription repression. Promotes transcription activation in differentiated myotubes (By similarity). Binds to double- and single-stranded DNA sequences. Binds to the transcription suppressor CATR sequence of the COX5B promoter (By similarity). Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Binds both to nuclear and cytoplasmic poly(A) mRNAs. Binds to poly(G) and poly(A), but not to poly(U) or poly(C) RNA homopolymers. Binds to the 5'-ACUAGC-3' RNA consensus sequence.

• 产品名称Anti-hnRNP DL antibody

• 分子量46 KDa

• 种属反应性Human,Mouse,Rat,Chicken,Dog,Cow,Sheep

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human hnRNP DL 101-200/420

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm in a TNPO1-dependent manner.

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• ​其它名称A+U-rich element RNA binding factor
  AA407431
  AA959857
  AU rich element RNA binding factor
  AU-rich element RNA-binding factor
  D5Ertd650e
  D5Wsu145e
  Heterogeneous nuclear ribonucleoprotein D like
  Heterogeneous nuclear ribonucleoprotein D like protein
  Heterogeneous nuclear ribonucleoprotein D-like
  hnHNRP DL
  HNRDL_HUMAN
  HNRNP
  hnRNP D-like
  hnRNP DL
  HNRNPDL
  hnRPD like protein
  JKT41 binding protein
  JKT41-binding protein
  JKTBP
  JKTBP2
  laAUF1
  MGC125262
  Protein laAUF1。

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

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  Fig1: Sample:
  Muscle (Mouse) Lysate at 40 ug
  Primary: Anti- hnRNP DL  at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 46 kD
  Observed band size: 48 kD

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (hnRNP DL) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！