Anti-CD11b antibody [A2-B5-D2]

Anti-CD11b antibody [A2-B5-D2]

• 概述

• 产品描述Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (By similarity). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (By similarity). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development .
• 产品名称Anti-CD11b antibody [A2-B5-D2]
• 分子量Predicted band size: 125 kDa.
• 种属反应性Human, Mouse
• 验证应用WB,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein within mouse CD11b aa 1-300.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgM
• 纯化方式Protein A/G purified.
• 亚细胞定位Cell membrane, Membrane.
• 其它名称
  • antigen CD11b (p170) antibody
  • Antigen CD11b p170 antibody
  • CD11 antigen like family member B antibody
  more

• 应用

  WB: 1:500
  FC: 1:50-1:100

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  Fig1: Western blot analysis of CD11b on Mouse spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig2: Flow cytometric analysis of CD11b was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa FluorTM488 Goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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