Anti-Cdc14A antibody

Anti-Cdc14A antibody

• 概述

• 产品描述Overproduction of hCdc14A leads to progressive cell death, accompanied by accumulation of pre-G1 DNA fragments and gradual elimination of cells at the G2/M transition. In 50% of the mitotic cells, multipolar mitotic spindles and misaggregated chromosomes are found. hCdc14A localizes to the centrosomes at the cell-cycle interphase stage. When the cell enters mitosis, this localization disappears suggesting that hCdc14A dissociates from the centrosomes at the G2/M transition. hCdc14A localizes to interphase centrosomes, but not to mitotic centrosomes, while hCdc14B localizes to the interphase nucleolus. Thus each isoform regulates separate cell cycle events. Cdc14A may affect cell cycle progression by its ability to dephosphorylate p53 at Ser315. This phosphatase activity is mediated by the interaction between the N-terminus of hCdc14A and the C-terminus of p53.

• 产品名称Anti-Cdc14A antibody

• 分子量67 KDa

• 种属反应性Human,Mouse,Rat,Dog,Cow,Horse,Sheep

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human Cdc14A 51-150/594

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Nucleus. Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle. Note=Centrosomal during interphase, released into the cytoplasm at the onset of mitosis. Subsequently localizes to the midzone of the mitotic spindle.

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• ​其它名称CDC 10
  Cdc 14
  Cdc 14A
  Cdc 14A1
  Cdc 14A2
  CDC10
  Cdc14
  CDC14 cell division cycle 14 homolog A
  CDC14 homolog A
  Cdc14A1
  Cdc14A2
  Cell division cycle 14 homolog A
  Dual specificity protein phosphatase CDC 14A
  Dual specificity protein phosphatase CDC14A
  hCDC14
  CC14A_HUMAN.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

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  Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Cdc14A) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig2: Sample:
  Hela(Human) Cell Lysate at 30 ug
  Primary: Anti-Cdc14A at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 67 kD
  Observed band size: 67 kD

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！