Anti-Prostaglandin E Receptor EP1 antibody

Anti-Prostaglandin E Receptor EP1 antibody

• 概述

• 产品名称Anti-Prostaglandin E Receptor EP1 antibody

• 分子量42 KDa

• 种属反应性Human,Mouse,Rat,Dog

• 验证应用WB,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human PTGER1/Prostaglandin E Receptor EP1 61-160/402

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cell membrane; Multi-pass membrane protein.

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• ​其它名称Prostaglandin E Receptor EP1
  EP1
  PGE receptor EP1 subtype
  PGE2 receptor EP1 subtype
  Prostaglandin E receptor 1 subtype EP1 42kDa
  Prostaglandin E receptor 1 subtype EP1
  Prostaglandin E2 receptor EP1 subtype
  Prostanoid EP1 receptor
  PE2R1_HUMAN.

  more

• 应用

  WB:1:500-2000
  FC:1ug/Test

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  Fig1: Sample: Kidney (Mouse) Lysate at 40 ug
  Primary: Anti-Prostaglandin E Receptor EP1  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 42 kD
  Observed band size: 45 kD

   

  

  Fig2: Sample: Brain (Rat) Lysate at 40 ug
  Primary: Anti-Prostaglandin E Receptor EP1  at 1/300 dilution
  Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
  Predicted band size: 42 kD
  Observed band size: 42 kD

   

  

  Fig3: Blank control (Black line): Mouse blood (Black).
  Primary Antibody (green line): Rabbit Anti-Prostaglandin E Receptor EP1 antibody (
  Dilution: 3μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
  Dilution: 1μg /test.
  Protocol
  The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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