Anti-CD62L antibody

Anti-CD62L antibody

• 概述

• 产品描述This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene.
• 产品名称Anti-CD62L antibody
• 分子量37 KDa
• 种属反应性Mouse,Rat,Cow,Rabbit
• 验证应用WB,IHC-P,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from mouse CD62L 301-372/372
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Membrane; Single-pass type I membrane protein.
• 其它名称L-Selectin
  A.11
  AI528707
  CD62 antigen ligand
  CD62 antigen-like family member L
  gp90 MEL
  hLHRc
  IgA nephropathy, susceptibility to, included
  L Selectin
  LAM1
  LECAM1
  LEU8
  Leukocyte adhesion molecule 1
  Leukocyte surface antigen Leu8
  Leukocyte-endothelial cell adhesion molecule 1
  LNHR
  LSEL
  Ly-22
  Ly-m22
  Lyam-1
  LYAM1
  Lymph node homing receptor
  Lymphocyte adhesion molecule 1
  Lymphocyte antigen 22
  Lymphocyte surface MEL-14 antigen
  PLNHR
  Selectin L
  Selectin, lymphocyte
  SELL
  TQ1
  LYAM1_MOUSE.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:1μg/Test

•  

  

  Fig1: Sample:
  Lymph node (Mouse) Lysate at 40 ug
  Lymph node (Rat) Lysate at 40 ug
  Primary: Anti- CD62L  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 37 kD
  Observed band size: 43 kD

   

  

  Fig2: Blank control: mouse spleen cells (blue).
  Primary Antibody: Rabbit Anti- CD62L antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
  Protocol
  The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (ER1905-95, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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