Anti-MAG antibody
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概述
- 产品描述Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid.
- 产品名称Anti-MAG antibody
- 分子量67 KDa
- 种属反应性Human,Mouse,Rat,Dog,Cow,Horse
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human MAG 551-626/626
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Membrane.
- 其它名称Myelin associated glycoprotein
L-MAG
brain-specific neuronal polypeptide 1B236
Myelin-associated glycoprotein
Sialic acid-binding Ig-like lectin 4a
Siglec-4a
Brain neuron cytoplasmic protein 3
MAG_HUMAN
GMA
MAG
S MAG
Siglec 4a
SIGLEC4A.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Sample:U251 Cell Lysate at 40 ug
Primary: Anti-MAGat 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size:67 kD
Observed band size:63 kD
Fig2: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MAG Polyclonal Antibody, Unconjugated 1:600, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
