Anti-PARP1 antibody
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概述
- 产品描述Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.
- 产品名称Anti-PARP1 antibody
- 分子量113 kDa
- 种属反应性Human,Mouse
- 验证应用WB,ICC,FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide within PARP1 aa 200-250.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Nucleus.
- 其它名称ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
ADP ribosyltransferase antibody
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ADPRT 1 antibody
ADPRT antibody
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ARTD1 antibody
msPARP antibody
NAD(+) ADP ribosyltransferase 1 antibody
NAD(+) ADP-ribosyltransferase 1 antibody
pADPRT 1 antibody
pADPRT1 antibody
PARP 1 antibody
PARP antibody
PARP-1 antibody
PARP1 antibody
PARP1_HUMAN antibody
Poly (ADP ribose) polymerase 1 antibody
poly (ADP ribose) polymerase family, member 1 antibody
Poly [ADP-ribose] polymerase 1 antibody Poly(ADP ribose) polymerase antibody
poly(ADP ribose) synthetase antibody
poly(ADP ribosyl)transferase antibody
Poly[ADP ribose] synthetase 1 antibody
Poly[ADP-ribose] synthase 1 antibody
PPOL antibody
more
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应用
WB: 1:500
ICC: 1:50-1:100
FC: 1:50-1:100
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Fig1: Western blot analysis of PARP1 on Daudi and 293 cell lysates using anti- PARP1 antibody at 1/500 dilution.
Fig2: ICC staining PARP1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining PARP1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Flow cytometric analysis of K562 cells with PARP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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