Anti-GRP94 antibody
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概述
- 产品描述Heat shock protein (HSP) molecular chaperones are environmental stress-inducible gene products. The human HSP 90 family includes 17 genes that fall into four classes: HSP90AA, HSP90AB, HSP90B and TRAP. HSP 90 family members guide the normal folding, intracellular disposition and proteolytic turnover of many key regulators of cell growth, differentiation and survival. HSP 90α, also designated HSP90A, HSP 86 and LPS-associated protein 2 (LAP2), is a cytosolic enhancer of inducible nitric-oxide synthase (iNOS), with chaperone activity that is important for the transcriptional activity of p53. HSP 90β, also designated HSP90B, HSP 84 and HSPC2, is a cytosolic protein that participates in signaling pathways with PKC e to protect cells from external damage, particularly in heat shock-mediated events. GRP 94, also known as tumor rejection antigen 1 (TRA1), ECGP and GP96, localizes to the ER, is highly expressed in BGC-823 human gastric carcinoma cells and is upregulated in human endothelial cells in response to hypoxia by HIF-1. TRAP1 (TNF receptor-associated protein 1), also designated HSP 75) is a mitochondrial matrix component that plays a role in the induction of apoptosis in response to reactive oxygen species.
- 产品名称Anti-GRP94 antibody
- 分子量94 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P
- 抗体类型兔多抗
- 免疫原Peptide.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Endoplasmic reticulum lumen. Melanosome.
- 其它名称94 kDa glucose regulated protein antibody
94 kDa glucose-regulated protein antibody
ECGP antibody
Endoplasmin antibody
Endothelial cell (HBMEC) glycoprotein antibody
ENPL_HUMAN antibody
Glucose regulated protein 94kDa antibody
gp96 antibody
gp96 homolog antibody
GRP 94 antibody
GRP-94 antibody
Heat shock protein 90 kDa beta member 1 antibody
heat shock protein 90kDa beta (Grp94), member 1 antibody
Heat shock protein, 90 kDa, beta, 1 antibody
HSP90B1 antibody
Stress inducible tumor rejection antigen GP96 antibody
TRA1 antibody
tumor rejection antigen (gp96) 1 antibody
Tumor rejection antigen 1 antibody
Tumor rejection antigen gp96 antibody
Tumor rejection antigen-1 (gp96) antibody
more
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应用
WB: 1:500-1:2,000
ICC: 1:100-1:500
IHC-P: 1:100-1:500
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Fig1: Western blot analysis of GRP94 on different cell lysate using anti-GRP94 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: NIH-3T3
Lane 3: PC-12
Fig2: ICC staining GRP94 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining GRP94 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining GRP94 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GRP94 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-GRP94 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-GRP94 antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-GRP94 antibody. Counter stained with hematoxylin.
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