Anti-Ack1 antibody
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概述
- 产品描述Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR.
- 产品名称Anti-Ack1 antibody
- 分子量119 KDa
- 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human Ack1 301-420/1055
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cell membrane. Nucleus. Endosome. Cell junction, adherens junction. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, clathrin-coated vesicle. Membrane, clathrin-coated pit. Note=The Tyr-284 phosphorylated f
- 其它名称Acetate kinase 1
Acetokinase 1
Activated CDC42 kinase 1
Activated Cdc42 associated kinase 1
Activated p21cdc42Hs kinase
Tyrosine kinase non receptor 2
Tyrosine kinase non-receptor protein 2
non-receptor protein tyrosine kinase ACK
ACK
TNK 2
Tnk2
ACK1
FLJ44758
FLJ45547
ACK 1
ACK-1
ACK1_HUMAN
Activated p21cdc42Hs kinase
FLJ44758
FLJ45547
Tyrosine kinase non receptor protein 2
p21cdc42Hs.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Ack1) Polyclonal Antibody, Unconjugatedat 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig2: Sample:
Spinal cord(Mouse)l Lysate at 40 ug
Primary: Anti-Ack1 at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 119 kD
Observed band size:119 kD
Fig3: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Ack1) Polyclonal Antibody, Unconjugated ( at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
