Anti-USP13 antibody
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概述
- 产品描述The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP13 (ubiquitin specific peptidase 13), also known as ISOT3 (Isopeptidase T-3), is an 863 amino acid protein that belongs to the peptidase C19 family and contains one UBP-type zinc finger and two UBA domains. Highly expressed in testicular and ovarian tissue, USP13 functions to catalyze the water-dependent conversion of a ubiquitin C-terminal thioester to a thiol and a free ubiquitin.
- 产品名称Anti-USP13 antibody
- 分子量97 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,FC,IHC-P
- 抗体类型兔多抗
- 免疫原Recombinant protein within human USP13 aa 550-800.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Nucleus. Cytosol.
- 其它名称Deubiquitinating enzyme 13 antibody
Isopeptidase T 3 antibody
Isopeptidase T-3 antibody
Isopeptidase T3 antibody
ISOT 3 antibody
ISOT-3 antibody
ISOT3 antibody
Ubiquitin carboxyl terminal hydrolase 13 antibody
Ubiquitin carboxyl-terminal hydrolase 13 antibody
Ubiquitin specific peptidase 13 (isopeptidase T3) antibody
Ubiquitin specific peptidase 13 antibody
Ubiquitin specific processing protease 13 antibody
Ubiquitin specific protease 13 antibody
Ubiquitin thioesterase 13 antibody
Ubiquitin thiolesterase 13 antibody
Ubiquitin-specific-processing protease 13 antibody
UBP13 antibody
UBP13_HUMAN antibody
USP 13 antibody
USP13 antibody
more
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应用
WB: 1:500-1:2,000
ICC: 1:50-1:200
FC: 1:50-1:100
IHC-P: 1:100-1:200
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Fig1: Western blot analysis of USP13 on different lysates using anti-USP13 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: Daudi
Lane 3: Mouse testis
Lane 4: Rat brain
Fig2: ICC staining USP13 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining USP13 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Flow cytometric analysis of A431 cells with USP13 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
