Anti-CPLX1 antibody
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概述
- 产品描述Positively regulates a late step in synaptic vesicle exocytosis. Also involved in glucose-induced secretion of insulin by pancreatic beta-cells.
- 产品名称Anti-CPLX1 antibody
- 分子量15 KDa
- 种属反应性Human,Mouse,Rat,Chicken,Pig,Cow
- 验证应用WB,IHC-P,ICC/IF
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human CPLX1 31-100/134
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cytoplasm; cytosol. Enriched at synaptic-releasing sites in mature neurons.
- 其它名称complexin 1
Complexin I
Complexin-1
CPLX1
CPLX1_HUMAN
CPX I
CPX-I
CPX1
Synaphin 2
Synaphin-2
921-S.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
IF:1:100-500
ICC/IF:1:100-500
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Fig1: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CPLX1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Fig2: Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CPLX1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary for 20 minutes and DAB staining.
Fig3: Sample:
RSC96(Rat) Cell Lysate at 30 ug
Primary: Anti- CPLX1 at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kD
Observed band size: 17 kD
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