Anti-Fyn antibody
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概述
- 产品描述Src is the human homolog of the v-src gene of the Rous sarcoma virus, also called avian sarcoma virus or ASV. Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Src-family kinases contain an amino terminal cell membrane anchor followed by an SH3 domain and an SH2 domain involved in modular association and activation, respectively. Src-family kinases are normally maintained in an inactive state and can be activated transiently during cellular events such as mitosis. Different subcellular localizations of Src-family kinases may be important for the regulation of specific cellular processes such as mitogenesis, cytoskeletal organization, and membrane trafficking. Fyn and Lck kinases play a key role in T-cell antigen receptor (TCR) signaling. The human Fyn gene maps to chromosome 6q21 and encodes a 537 amino acid protein.
- 产品名称Anti-Fyn antibody
- 分子量60 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Cytoplasm. Membrane. Nucleus.
- 其它名称C syn protooncogene antibody
Fyn antibody
FYN oncogene related to SRC FGR YES antibody
FYN_HUMAN antibody
OKT3 induced calcium influx regulator antibody
P59 FYN antibody
p59-Fyn antibody
Protein tyrosine kinase fyn antibody
Proto oncogene tyrosine protein kinase fyn antibody
Proto-oncogene c-Fyn antibody
Proto-oncogene Syn antibody
Protooncogene Syn antibody
SLK antibody
Src like kinase antibody
Src yes related novel gene antibody
Src-like kinase antibody
Src/yes related novel antibody
SYN antibody
Tyrosine kinase p59fyn T antibody
Tyrosine kinase p59fyn(T) antibody
Tyrosine-protein kinase Fyn antibody
more
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应用
WB: 1:500
ICC: 1:100-1:500
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of Fyn on HUVEC cell using anti-Fyn antibody at 1/200 dilution.
Fig2: ICC staining Fyn in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Fyn in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Fyn in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Fyn antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Fyn antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Fyn antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Fyn antibody. Counter stained with hematoxylin.
Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Fyn antibody. Counter stained with hematoxylin.
Fig10: Flow cytometric analysis of SH-SY5Y cells with Fyn antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the second
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