Anti-DDX5 antibody [C12-F1]

Anti-DDX5 antibody [C12-F1] 

• 概述

• 产品描述p68 RNA helicase (also named DDX5) is a nuclear protein that exhibits RNA-dependent ATPase activity. Phosphorylation by protein kinase C inhibits p68 RNA helicase activity. p68 RNA helicase appears to play a role in organ differentiation during development. Furthermore, p68 RNA helicase is expressed in early neural development and in various mesodermal tissues in a number of different chordate embryos. At the cellular level, the expression levels of p68 RNA helicase increases in serum-induced quiescent cell lines. p68 RNA helicase may function as a coactivator for estrogen receptor alpha. Additionally, p68 RNA helicase associates with transcriptional coactivators CBP and p300. p68 RNA helicase localizes to the nucleus under normal conditions. During late telophase, p68 RNA helicase and fibrillarin colocalize to nascent nucleoli. p68 RNA helicase may function as a heterodimer with p72 RNA helicase.
• 产品名称Anti-DDX5 antibody [C12-F1]
• 分子量69 kDa
• 种属反应性Human,Mouse,Monkey
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2a
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus.
• 其它名称
  • ATP dependent RNA helicase DDX5 antibody
  • DDX 5 antibody
  • Ddx5 antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of DDX5 on human DDX5 recombinant protein using anti-DDX5 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of DDX5 on HEK293 (1) and DDX5-hIgGFc transfected HEK293 (2) cell lysate using anti-DDX5 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of DDX5 on different cell lysate using anti-DDX5 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: HT-29
  Lane 2: Hela
  Lane 3: NIH/3T3
  Lane 4: COS7
  Lane 5: SW620
  Lane 6: Jurkat
  Lane 7: A431
  Lane 8: MCF-7

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-DDX5 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-DDX5 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of Hela cells with DDX5 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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