-
专业包装 正品保证
-
快乐服务 售后无忧
-
会员特权 优惠不断
-
个人信息 严格保护
| 别名: | CD30-L,CD153,TNFSF8,CD30L,CD30LG,CD153 antigen,CD30 antigen ligand,CD30 Ligand | ||
|---|---|---|---|
| 储存条件: | -20℃ | ||
|
| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
|---|
| 熔点: | |
|---|---|
| 密度: | |
| 储存条件: | -20℃ |
Human CD30 Ligand / TNFSF8 Protein, His Tag, low endotoxin (MALS verified)
CD30-L,CD153,TNFSF8,CD30L,CD30LG,CD153 antigen,CD30 antigen ligand,CD30 Ligand
Human CD30 Ligand, His Tag, low endotoxin is expressed from human 293 cells. It contains AA Gln 63 - Asp 234 .
Predicted N-terminus: His
Request for sequence
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 21.5 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 0.01 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human CD30 Ligand, His Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human CD30 Ligand, His Tag, low endotoxins more than 95% and the molecular weight of this protein is around 61-83 kDa verified by SEC-MALS.
Report
Immobilized Human CD30, Fc Tag at 2 μg/mL (100 μL/well) can bind Human CD30 Ligand, His Tag, low endotoxinwith a linear range of 0.039-0.313 μg/mL (QC tested).
Protocol
Immobilized Human CD30 Ligand, His Tag, low endotoxin at 2 μg/mL (100 μL/well) can bind Human CD30, Llama IgG2b Fc Tag, low endotoxin with a linear range of 0.6-10 ng/mL (Routinely tested).
Protocol
Loaded Biotinylated Human CD30, Avitag,His Tagon SA Biosensor, can bind Human CD30 Ligand, His Tag, low endotoxin with an affinity constant of 132 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
FACS analysis shows that Human CD30 Ligand, His Tag, low endotoxincan bind to K562-CD30 cells surface CD30. The concentration of Human CD30 Ligand is 3 μg/mL (Routinely tested).
Protocol
FACS analysis shows that the binding of Human CD30 Ligand, His Tag, low endotoxin to K562-CD30 cells surface CD30 was inhibited by increasing concentration of neutralizing anti-CD30 antibody. The concentration of Human CD30 Ligand used is 3 μg/mL. The IC50 is 0.2401 μg/mL (Routinely tested).
