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Human Complement C2 Protein, His Tag (MALS verified) (active enzyme)

Human Complement C2 Protein, His Tag (MALS verified) (active enzyme)
别名: C2,Complement C2,Complement Component C2
储存条件: -20℃ 
Human Complement C2 Protein, His Tag (MALS verified) (active enzyme)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-CO2-H52H7-50ug 50ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

Human Complement C2 Protein, His Tag (MALS verified) (active enzyme)

分子别名(Synonym)

C2,Complement C2,Complement Component C2

表达区间及表达系统(Source)

Human Complement C2, His Tag  is expressed from human 293 cells. It contains AA Ala 21 - Leu 752 .

Predicted N-terminus: Ala 21

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 83.0 kDa. The protein migrates as 95-110 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human Complement C2, His Tag (Cat. No. CO2-H52H7) SDS-PAGE gel

Human Complement C2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

The purity of Human C2, His Tag  is more than 90% and the molecular weight of this protein is around 100-120 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB).The specific activity is >100 pmol/min/µg (QC tested).

 

 

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