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| 别名: | CD40LG,CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39 | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human CD40 Ligand / TNFSF5 Protein, His,Flag Tag (active trimer) (MALS verified)
CD40LG,CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39
Human CD40 Ligand, His,Flag Tag is expressed from human 293 cells. It contains AA Gly 116 - Leu 261 trimer Design.
Predicted N-terminus: His
This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.
The protein has a calculated MW of 49.9 kDa. The protein migrates as 45-55 kDa under non-reducing (NR) condition (SDS-PAGE).
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human CD40 Ligand, His,Flag Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human CD40 Ligand, His,Flag Tagis more than 85% and the molecular weight of this protein is around 51-69 kDa verified by SEC-MALS.
Report
Immobilized Human CD40, Fc Tag at 5 μg/mL (100 μL/well) can bind Human CD40 Ligand, His,Flag Tag with a linear range of 20-156 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human CD40, Fc,Avitag at 5 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Human CD40 Ligand, His,Flag Tagwith a linear range of 0.039-1.25 μg/mL (Routinely tested).
Protocol
Loaded Human CD40, Fc Tag on Protein A Biosensor, can bind Human CD40 Ligand, His,Flag Tag with an affinity constant of 1.58 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Human CD40 Ligand, His,Flag Tag stimulates secretion of IL-6 by human B cells. The ED50 for this effect is 18.76-19.45 ng/mL in the presence of 5 ng/mL of Recombinant Human IL‑4 (Routinely tested).
Protocol
Neutralization assay shows that the cytokine secretion effect of Human CD40 Ligand, His,Flag Tag was inhibited by increasing concentration of anti-CD40L mAb. The concentration of CD40L used is 100 ng/mL. The EC50 is 0.215 μg/mL (Routinely tested).
