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Human CTGF / CCN2 Protein, His Tag

Human CTGF / CCN2 Protein, His Tag
别名: CCN2,NOV2,HCS24,IGFBP8,IBP-8,IGFBP-8,IGF-binding protein 8
储存条件: -20℃ 
Human CTGF / CCN2 Protein, His Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-CTF-H52H5-100ug 100ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

Human CTGF / CCN2 Protein, His Tag

分子别名(Synonym)

CCN2,NOV2,HCS24,IGFBP8,IBP-8,IGFBP-8,IGF-binding protein 8

表达区间及表达系统(Source)

Human CTGF, His Tag  is expressed from human 293 cells. It contains AA Gln 27 - Ala 349 .

Predicted N-terminus: Gln 27

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 37.4 kDa. The protein migrates as 36-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Potassium glutamate and Arginine, pH7.0. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human CTGF, His Tag (Cat. No. CTF-H52H5) SDS-PAGE gel

Human CTGF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CTGF, His Tag  at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CTGF Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Monoclonal Anti-Human CTGF Antibody, Human IgG1 on AHC Biosensor, can bind Human CTGF, His Tagwith an affinity constant of 6.29 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 

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