Human EGF R Protein, His Tag (MALS verified)

Human EGF R Protein, His Tag (MALS verified)

分子别名（Synonym）

EGFR,ERBB,ERBB1,HER1,PIG61,mENA

表达区间及表达系统（Source）

Human EGF R, His Tag  expressed from human 293 cells  It contains AA Leu 25 - Ser 645 .

Predicted N-terminus: Leu 25

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 70.5kDa. The protein migrates as 95-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 6 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Human EGF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Human EGF R, His Tag is more than 90% and the molecular weight of this protein is around 80-105 kDa verified by SEC-MALS.

Report

 

活性（Bioactivity）-ELISA

Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind Human EGF R, His Tag with a linear range of 0.006-0.025 μg/mL (QC tested).

Protocol

Report

活性（Bioactivity）-SPR

Erbitux (Cetuximab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human EGF R, His Tag with an affinity constant of 1.3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

Human EGF R, His Tag captured on CM5 Chip via anti-His antibody can bind Human EGF, Mouse IgG2a Fc Tag with an affinity constant of 2.63 nM as determined in SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性（Bioactivity）-BLI

Loaded Erbitux (Cetuximab) on AHC Biosensor, can bind Human EGF R, His Tag  with an affinity constant of 1.23 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

活性（Bioactivity）-FACS

2e5 of Anti-EGFR CAR-293 cells were stained with 100 μL of 1 μg/mL of Human EGF R, His Tag and negative control protein respectively, washed and then followed by PE anti-His antibody and analyzed with FACS (Routinely tested).

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