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Mouse IL-1 beta / IL-1F2 Protein, His Tag (MALS verified)

Mouse IL-1 beta / IL-1F2 Protein, His Tag (MALS verified)
别名: IL1B,IL-1BETA,IL1F2,IL-1β
储存条件: -20℃ 
Mouse IL-1 beta / IL-1F2 Protein, His Tag (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-ILB-M51H3-5 20μg 现货 0.00
ZY-ILB-M51H3-20ug 20μg 现货 1600.00 1600
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熔点:
密度:
储存条件: -20℃

 

Mouse IL-1 beta / IL-1F2 Protein, His Tag (MALS verified)

分子别名(Synonym)

IL1B,IL-1BETA,IL1F2,IL-1β

表达区间及表达系统(Source)

Mouse IL-1 beta, His Tag is expressed from E.coli cells. It contains AA Val 118 - Ser 269 .

Predicted N-terminus: Met

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 19.3 kDa. The protein migrates as 19-20 kDa under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Mouse IL-1 beta, His Tag (Cat. No. ILB-M51H3) SDS-PAGE gel

Mouse IL-1 beta, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Mouse IL-1 beta, His Tags more than 85% and the molecular weight of this protein is around 20-25 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Mouse IL-1 beta, His Tag  at 10 μg/mL (100 μL/well) can bind Human IL-1 RII, Fc Tag with a linear range of 20-156 ng/mL (QC tested).

 

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