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| 别名: | IL-2 R beta & IL-2 R alpha & IL-2 R gamma | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Rat IL-2 R beta&IL-2 R alpha&IL-2 R gamma Protein, His Tag&Twin-Strep Tag (MALS verified)
IL-2 R beta & IL-2 R alpha & IL-2 R gamma
Rat IL-2RB&IL-2RA&IL-2RG, His Tag&Twin-Strep Tag is expressed from human 293 cells. It contains AA Ala 27 - Glu 239 (IL-2RB) & Glu 22 - Gln 235 (IL-2RA) & Ser 25 - Ala 262 (IL-2RG).
Predicted N-terminus: Ala 27 (IL-2RB) & Glu 22 (IL-2RA)
Rat IL-2RB&IL-2RA&IL-2RG, His Tag&Twin-Strep Tag is produced by co-expression of IL-2RB and IL-2RA and IL-2RG, has a calculated MW of 30.2 kDa (IL-2RB) and 59.3 kDa (IL-2RA&IL-2RG). Subunit IL-2RB is fused with a his tag at the C-terminus and subunit IL-2RA&IL-2RG is fused with a Twin-Strep Tag at the C-terminus. The reducing (R) protein migrates as 45 kDa and 110 kDa respectively due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by reduced SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Rat IL-2RB&IL-2RA&IL-2RG, His Tag&Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Rat IL-2RB&IL-2RA&IL-2RG, His Tag&Twin-Strep Tag is more than 85% and the molecular weight of this protein is around 125-145 kDa verified by SEC-MALS.
Report
Immobilized Biotinylated Human IL-2, Fc,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Rat IL-2RB&IL-2RA&IL-2RG, His Tag&Twin-Strep Tag with a linear range of 39-156 ng/mL (QC tested).
Protocol
Loaded Rat IL-2RB&IL-2RA&IL-2RG, His Tag&Twin-Strep Tag on NTA Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 1.16 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
