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| 别名: | MET,AUTS9,HGFR,RCCP2,c-Met | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Biotinylated Human HGF R / c-MET Protein, Avitag™,His Tag (MALS verified)
MET,AUTS9,HGFR,RCCP2,c-Met
Biotinylated Human HGF R, Avitag,His Tag is expressed from human 293 cells. It contains AA Glu 25 - Thr 932.
Predicted N-terminus: Glu 25 (α chain) & Ser 308 (β chain)
Request for sequence
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 32.5 kDa (α chain) & 72.7 kDa (β chain). The protein migrates as 40-50 kDa (α subunit) and 90-100 kDa (β subunit) under reducing (R) condition (SDS-PAGE) due to glycosylation.
The mature form of HGFR is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the HABA assay / binding ELISA.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human HGF R, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Biotinylated Human HGF R, Avitag,His Tags more than 85% and the molecular weight of this protein is around 113-138 kDa verified by SEC-MALS.
Report
Immobilized Human HGF, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human HGF R, Avitag,His Tag with a linear range of 0.01-0.313 μg/mL (QC tested).
