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| 别名: | RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 密度: | |
| 储存条件: | -20℃ |
Biotinylated Mouse VEGF164 Protein, His,Avitag™
RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF
Biotinylated Mouse VEGF164, His,Avitag is expressed from human 293 cells. It contains AA Ala 27 - Arg 190
Predicted N-terminus: His
Request for sequence
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 22.5 kDa. As a result of different glycosylation, the protein migrates as 28-33 kDa under reducing (R) condition, and 46-60 kDa under non-reducing (NR) condition (SDS-PAGE).
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the HABA assay / binding ELISA.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Mouse VEGF164, His,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized VEGFR1/R2, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse VEGF164, His,Avitag with a linear range of 0.02-0.5 ng/mL (QC tested).
Protocol
Immobilized Human VEGF R1, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse VEGF164, His,Avitag with a linear range of 0.2-2 ng/mL (Routinely tested).
Protocol
Loaded Biotinylated Mouse VEGF164, His,Avitag on SA Biosensor, can bind Mouse VEGF R1, Mouse IgG2a Fc Tag, low endotoxin with an affinity constant of 42.4 pM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Mouse VEGF164, His,Avitag on SA Biosensor, can bind Mouse VEGF R2, Mouse IgG2a Fc Tag, low endotoxin with an affinity constant of 1.33 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
