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| 别名: | FGF-7,Fibroblast growth factor 7,HBGF-7,Keratinocyte growth factor,KGF | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Biotinylated Human FGF-7 / HBGF-7 / KGF Protein, His,Avitag™ (MALS verified)
FGF-7,Fibroblast growth factor 7,HBGF-7,Keratinocyte growth factor,KGF
Biotinylated Human FGF-7, His,Avitag is expressed from human 293 cells . It contains AA Cys 32 - Thr 194.
Predicted N-terminus: Cys 32
Request for sequence
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 22.6 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the HABA assay / binding ELISA.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human FGF-7, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human FGF-7, His,Avitag is more than 90% and the molecular weight of this protein is around 31-47 kDa verified by SEC-MALS.
Report
Loaded Biotinylated Human FGF-7, His,Avitagon SA Biosensor, can bind Human FGF R2 (IIIb), Fc Tag with an affinity constant of 37 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
Loaded Biotinylated Human FGF-7, His,Avitag on SA Biosensor, can bind Human FGF R1, His Tag with an affinity constant of 243 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
