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| 别名: | IFN-alpha 1,IFNA1,IFNalpha 1,IFN-alpha-1,LeIF D | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human IFN-alpha 1 Protein, Fc Tag (MALS verified)
IFN-alpha 1,IFNA1,IFNalpha 1,IFN-alpha-1,LeIF D
Human IFN-alpha 1, Fc Tags expressed from human 293 cells . It contains AA Cys 24 - Glu 189
Predicted N-terminus: Cys 24
Request for sequence
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 45.8 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 51 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human IFN-alpha 1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human IFN-alpha 1, Fc Tag is more than 90% and the molecular weight of this protein is around 90-118 kDa verified by SEC-MALS.
Report
Loaded Human IFN-alpha / beta R1, His Tag on NTA Biosensor, can bind Human IFN-alpha 1, Fc Tag with an affinity constant of 11.3 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
Loaded Human IFNAR2, His Tagon HIS1K Biosensor, can bind Human IFN-alpha 1, Fc Tag with an affinity constant of 2.67 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
