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Human LAP (TGF-beta 1) Protein, Tag Free (MALS verified)

Human LAP (TGF-beta 1) Protein, Tag Free (MALS verified)
别名: LAP (TGF-beta 1),LAP (TGFB1),TGFB1,CED,DPD1,LAP,TGF-beta-1,TGFB
储存条件: -20℃ 
Human LAP (TGF-beta 1) Protein, Tag Free (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-LAP-H5213-100ug 100ug 现货 4100.00 4100
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熔点:
密度:
储存条件: -20℃

 

Human LAP (TGF-beta 1) Protein, Tag Free (MALS verified)

 

分子别名(Synonym)

LAP (TGF-beta 1),LAP (TGFB1),TGFB1,CED,DPD1,LAP,TGF-beta-1,TGFB

表达区间及表达系统(Source)

Human LAP (TGF-beta 1), Tag Freeis expressed from human 293 cells . It contains AA Leu 30 - Arg 278 

Predicted N-terminus: Leu 30

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries no "tag".

The protein has a calculated MW of 28.5 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human LAP (TGF-beta 1), Tag Free (Cat. No. LAP-H5213) SDS-PAGE gel

Human LAP (TGF-beta 1), Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

The purity of Human LAP (TGF-beta 1), Tag Free is more than 85% and the molecular weight of this protein is around 62-76 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human LAP (TGF-beta 1), Tag Free at 1 μg/mL (100 μL/well) can bind Human ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Freewith a linear range of 5-39 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human LAP (TGF-beta 1), Tag Free at 1 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free  with a linear range of 1-39 ng/mL (Routinely tested).

 

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