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Rat 4-1BB Ligand / TNFSF9 Protein, His,Flag Tag (MALS verified)

Rat 4-1BB Ligand / TNFSF9 Protein, His,Flag Tag (MALS verified)
别名: 4-1BB Ligand,TNFSF9,CD137L
储存条件: -20℃ 
Rat 4-1BB Ligand / TNFSF9 Protein, His,Flag Tag (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-41L-R52D3-100ug 100ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

Rat 4-1BB Ligand / TNFSF9 Protein, His,Flag Tag (MALS verified)

分子别名(Synonym)

4-1BB Ligand,TNFSF9,CD137L

表达区间及表达系统(Source)

Rat 4-1BB Ligand His,Flag Tag  is expressed from human 293 cells . It contains AA Pro 106 - Gly 308.

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.

The protein has a calculated MW of 72.3 kDa. The protein migrates as 95-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Rat 4-1BB Ligand His,Flag Tag (Cat. No. 41L-R52D3) SDS-PAGE gel

Rat 4-1BB Ligand His,Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Rat 4-1BB Ligand His,Flag Tag is more than 90% and the molecular weight of this protein is around 169-206 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Mouse 4-1BB, Fc Tag  at 1 μg/mL (100 μL/well) can bind Rat 4-1BB Ligand His,Flag Tag  with a linear range of 5-39 ng/mL (QC tested).

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