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Human HGF R / c-MET Protein, His Tag

Human HGF R / c-MET Protein, His Tag
别名: MET,AUTS9,HGFR,RCCP2,c-Met
储存条件: -20℃ 
Human HGF R / c-MET Protein, His Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-MET-H5227-200ug 200ug 现货 3800.00 3800
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熔点:
密度:
储存条件: -20℃

Human HGF R / c-MET Protein, His Tag

分子别名(Synonym)

MET,AUTS9,HGFR,RCCP2,c-Met

表达区间及表达系统(Source)

Human HGF R, His Tag  is expressed from human 293 cells . It contains AA Glu 25 - Thr 932 .

Predicted N-terminus: Glu 25

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus. The mature form of HGFR is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 32.5 kDa and 70.2 kDa. The protein migrates as 40-45 kDa and 85-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human HGF R, His Tag (Cat. No. MET-H5227) SDS-PAGE gel

Human HGF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

获取HPLC数据

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human HGF/Hepatocyte Growth Factor at 2 μg/mL (100 μL/well) can bind Human HGF R, His Tag with a linear range of 0.039-0.313 μg/mL (Routinely tested).

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