Anti-PGRMC1 antibody [G8-G7]

Anti-PGRMC1 antibody [G8-G7]

• 概述

• 产品描述PGRMC1 (progesterone receptor membrane component 1), also known as MPR, is a 195 amino acid single-pass membrane protein that localizes to both the endoplasmic reticulum and to the microsome and contains one cytochrome b5 heme-binding domain. Expressed in a variety of tissues with highest expression in kidney and liver, PGRMC1 functions as a receptor for progesterone, a steroid hormone that is involved in embryonic development and is crucial for proper female maturation. The gene encoding PGRMC1 maps to human chromosome X, which contains nearly 153 million base pairs and houses over 1,000 genes. In conjunction with chromosome Y, chromosome X is responsible for sex determination, as an X and a Y chromosome lead to normal male development, while two copies of an X chromosome lead to normal female development. There are a number of conditions related to an abnormal number and combination of sex chromosomes, some of which include Turner's syndrome, color blindness, hemophilia and Duchenne muscular dystrophy
• 产品名称Anti-PGRMC1 antibody [G8-G7]
• 分子量22 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Microsome membrane. Endoplasmic reticulum membrane.
• 其它名称
  • HPR6.6 antibody
  • Membrane associated progesterone receptor component 1 antibody
  • Membrane-associated progesterone receptor component 1 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:200

•  

  

  Fig1: Western blot analysis of PGRMC1 on human PGRMC1 recombinant protein using anti-PGRMC1 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of PGRMC1 on HEK293 (1) and PGRMC1-hIgGFc transfected HEK293 (2) cell lysate using anti-PGRMC1 antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining PGRMC1 (green) and Actin filaments (red) in SK-OV-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-PGRMC1 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-PGRMC1 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of A549 cells with PGRMC1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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