Anti-SIRT1 antibody
-
概述
- 产品描述SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53, p300, Ku70, forkhead (FoxO) transcription factors, PPARγ, and the PPARγ coactivator-1α (PGC-1α) protein. Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival. SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol.
- 产品名称Anti-SIRT1 antibody
- 分子量110 kDa
- 种属反应性Human,Mouse
- 验证应用WB,IHC-P,FC
- 抗体类型兔多抗
- 免疫原peptide
- 偶联Non-conjugated
-
性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Nucleus, cytoplasm
- 其它名称75SirT1 antibody
hSIR2 antibody
hSIRT1 antibody
HST2, S. cerevisiae, homolog of antibody
NAD dependent deacetylase sirtuin 1 antibody
NAD dependent protein deacetylase sirtuin 1 antibody
OTTHUMP00000198111 antibody
OTTHUMP00000198112 antibody
Regulatory protein SIR2 homolog 1 antibody
SIR1_HUMAN antibody
SIR2 antibody
SIR2 like 1 antibody
SIR2 like protein 1 antibody
SIR2, S.cerevisiae, homolog-like 1 antibody
SIR2-like protein 1 antibody
SIR2ALPHA antibody
SIR2L1 antibody
Sirt1 antibody
SirtT1 75 kDa fragment antibody
Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) antibody
Sirtuin 1 antibody
Sirtuin type 1 antibody
more
-
应用
WB: 1:1,000-1:2,000
IHC-P: 1:200
FC: 1:100-1:200
-
Fig1: Western blot analysis of SIRT1 on different cell lysates using anti- SIRT1 antibody at 1/1000 dilution.
Positive control:
Lane 1: Hela
Lane 2: F9
Lane 3: Jurkat
Fig2: Flow cytometric analysis of Hela cells with SIRT1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-SIRT1 antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-SIRT1 antibody. Counter stained with hematoxylin.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
