Anti-TLR9 antibody [H2-B12]

Anti-TLR9 antibody [H2-B12]

• 概述

• 产品描述The Toll-like Receptors (TLR) are a family of human receptors that share homology with the Drosophila Toll Receptors, which are involved in mediating dorsoventral polarization in developing Drosophila embryos and participate in host immunity. The TLR family members are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transductions. TLRs are type I transmembrane receptors that contain an extracellular domain consisting of several leucine-rich regions and a single cytoplasmic Toll/IL-1R like domain. Three TLR family members, TLR7, TLR8 and TLR9, belong to a subfamily of TLRs which are differentially expressed. TLR7 is expressed in lung, placenta and spleen. TLR8 is expressed in lung and peripheral blood leukocytes, and TLR9 is predominantly expressed in spleen, lymph nodes, bone marrow and peripheral blood leukocytes. TLR7, TLR8 and TLR9 stimulate the NFκB signaling pathway, suggesting that they play a role in the immune response.
• 产品名称Anti-TLR9 antibody [H2-B12]
• 分子量116 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2a
• 纯化方式Protein A purified.
• 亚细胞定位Endoplasmic reticulum membrane. Cell membrane
• 其它名称
  • CD 289 antibody
  • CD289 antibody
  • TLR 9 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:200

•  

  

  Fig1: Western blot analysis of TLR9 on human TLR9 recombinant protein using anti-TLR9 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of TLR9 on HEK293 (1) and TLR9-hIgGFc transfected HEK293 (2) cell lysate using anti-TLR9 antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining TLR9 (green) and Actin filaments (red) in SK-OV-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-TLR9 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-TLR9 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of A549 cells with TLR9 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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