Anti-MRPL42 antibody [G11-H6]

Anti-MRPL42 antibody [G11-H6]

• 概述

• 产品描述Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q.

• 产品名称Anti-MRPL42 antibody [G11-H6]

• 分子量16 kDa

• 种属反应性Human

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型小鼠单抗

• 免疫原Recombinant protein

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度2 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG1

• 纯化方式Protein A purified.

• 亚细胞定位Mitochondrion.

• 数据链接Q9Y6G3 Human
  

• 其它名称

  more

  • 28S ribosomal protein S32 antibody

  • 39S ribosomal protein L31 antibody

  • 39S ribosomal protein L42 antibody

• 应用

  WB: 1:500-1:1,000
  ICC: 1:100-1:200
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of MRPL42 on human MRPL42 recombinant protein using anti-MRPL42 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of MRPL42 on HEK293 (1) and MRPL42-hIgGFc transfected HEK293 (2) cell lysate using anti-MRPL42 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of MRPL42 on different cell lysate using anti-MRPL42 antibody at 1/1,000 dilution.
  Positive control:
  Line1:HL7702 Line2:HepG2 Line3:SMMC-7721
  Line4:HEK293 Line5:HeLa Line6:Raji

   

  

  Fig4: ICC staining MRPL42 (green) in HeLa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-MRPL42 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-MRPL42 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of HepG2 cells with MRPL42 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

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