Anti-FAM3A antibody [7-B9]
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概述
- 产品描述The FAM3 gene family is a novel cytokine-like gene family, which includes four members designated as FAM3A, FAM3B, FAM3C, and FAM3D. FAM3A is a secreted protein belonging to the FAM3 family of four alpha -helix bundle cytokines. It is expressed in multiple tissues. Mature human FAM3A shares approximately 93% amino acid sequence identity with the mouse and rat protein.FAM3A belongs to a novel cytokine-like gene family, and its physiological role remains largely unknown. FAM3A activated Akt through the p110α catalytic subunit of PI3K in an insulin-independent manner. Blockade of P2 ATP receptors or downstream phospholipase C (PLC) and IP3R and removal of medium calcium all significantly reduced FAM3A-induced increase in cytosolic free Ca2+ levels and attenuated FAM3A-mediated PI3K/Akt activation. Moreover, FAM3A-induced Akt activation was completely abolished by the inhibition of calmodulin (CaM).
- 产品名称Anti-FAM3A antibody [7-B9]
- 分子量25 kDa
- 种属反应性Mouse,Rat,Human
- 验证应用WB,IHC-P,FC
- 抗体类型小鼠单抗
- 免疫原Recombinant protein.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度2 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG1
- 纯化方式Protein A purified.
- 亚细胞定位Secreted.
- 其它名称
- 2-19 antibody
- 2.19 antibody
- Cytokine-like protein 2-19 antibody
more
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应用
WB: 1:2,000-1:5,000
IHC-P: 1:50-1:200
FC: 1:50-1:200
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Fig1: Western blot analysis of FAM3A on recombinant protein using anti-FAM3A antibody at 1/1000 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-FAM3A antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-FAM3A antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-FAM3A antibody. Counter stained with hematoxylin.
Fig5: Flow cytometric analysis of A431 cells with FAM3A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
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