Anti-DCTN4 antibody [D7-G9]

Anti-DCTN4 antibody [D7-G9]

• 概述

• 产品描述Dynactin is a multisubunit complex and a required cofactor for most, or all, of the cellular processes powered by the microtubule-based motor cytoplasmic dynein. Dynactin contains a short actin-related protein 1 (Arp1) filament with capZ at the barbed end and p62 at the pointed end. The p62 subunit is an integral component of 20 S dynactin with a highly conserved cysteine-rich motif that interacts directly with Arp1. Dynactin p62 has a punctate cytoplasmic distribution as well as centrosomal distribution typical of dynactin. In addition, Dynactin p62 is distributed in the nucleus at very high expression levels. Due to the structural composition of dynactin, the p62 subunit is implicated in Arp1 pointed-end binding and in linking dynein and dynactin to the cortical cytoskeleton.
• 产品名称Anti-DCTN4 antibody [D7-G9]
• 分子量52 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm, cytoskeleton, centrosome.
• 其它名称
  • DCTN 4 antibody
  • Dctn4 antibody
  • DCTN4_HUMAN antibody
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:100-1:500
  IHC-P: 1:100-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of DCTN4 on human DCTN4 recombinant protein using anti-DCTN4 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of DCTN4 on HEK293 (1) and DCTN4-hIgGFc transfected HEK293 (2) cell lysate using anti-DCTN4 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of DCTN4 on Raw264.7 (1) and NIH3T3 (2) cell lysate using anti-DCTN4 antibody at 1/1,000 dilution.

   

  

  Fig4: ICC staining DCTN4 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-DCTN4 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-DCTN4 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of A549 cells with DCTN4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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