Anti-ZFP91 antibody [D5-C3]

Anti-ZFP91 antibody [D5-C3]

• 概述

• 产品描述The protein encoded by ZFP91 is a member of the zinc finger family of proteins. This protein contains C2H2 type domains, which are the classical zinc finger domains found in numerous nucleic acid-binding proteins. Atypical E3 ubiquitin-protein ligase that mediates 'Lys-63'-linked ubiquitination of MAP3K14/NIK, leading to stabilize and activate MAP3K14/NIK. It thereby acts as an activator of the non-canonical NF-kappa-B2/NFKB2 pathway. May also play an important role in cell proliferation and/or anti-apoptosis.
• 产品名称Anti-ZFP91 antibody [D5-C3]
• 分子量63 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus.
• 其它名称
  • FKSG11 antibody
  • HGNC:14983 antibody
  • PZF antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:100-1:200
  IHC-P: 1:100-1:500
  FC: 1:50-1:200

•  

  

  Fig1: Western blot analysis of ZFP91 on human ZFP91 recombinant protein using anti-ZFP91 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of ZFP91 on HEK293 (1) and ZFP91-hIgGFc transfected HEK293 (2) cell lysate using anti-ZFP91 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of ZFP91 on different cell lysate using anti-ZFP91 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: Jurkat
  Lane 2: A431
  Lane 3: HepG2
  Lane 4: HEK293
  Lane 5: A549
  Lane 6: PC-3

   

  

  Fig4: ICC staining ZFP91 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-ZFP91 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-ZFP91 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of Hela cells with ZFP91 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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