Anti-Hsp90 alpha antibody [A10-A5-D2]

Anti-Hsp90 alpha antibody [A10-A5-D2]

• 概述

• 产品描述The heat shock response was first described for Drosophila salivary gland cells and morphologically consists of a change in their polytene chromosome puffing patterns that involves de novo synthesis of a few proteins. Similar heat shock proteins were later discovered in bacterial chicken and mammalian cells, and have been subsequently studied in other organisms. A series of proteins including HSP 90, HSP 70, HSP 20-30 and ubiquitin are induced by insults such as temperature shock, chemicals and other environmental stress. A major function of HSP 90 and other HSPs is to act as molecular chaperones. HSP 90 forms a complex with glucocorticoid receptor (GR), rendering the non ligand-bound receptor transcriptionally inactive. HSP 90 binds the GR as a heterocomplex composed of either HSP 56 or Cyclophilin D, forming an aporeceptor comiplex. HSP 90 also exists as a dimer with other proteins such as p60/sti1 and p23, forming an apo-receptor complex with estrogen and androgen receptors.
• 产品名称Anti-Hsp90 alpha antibody [A10-A5-D2]
• 分子量90kDa
• 种属反应性Human, Mouse, Rat
• 验证应用WB, ICC, IHC-P, FC
• 抗体类型小鼠单抗
• 免疫原peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Peptide affinity purified
• 亚细胞定位Cytoplasm, Melanosome, Cell membrane
• 其它名称
  • EL52 antibody
  • epididymis luminal secretory protein 52 antibody
  • Heat shock 86 kDa antibody
  more

• 应用

  WB: 1:1,000-1:2,000
  ICC: 1:100-1:500
  IHC-P: 1:50-1:200
  FC: 1:50-1:200

•  

  

  Fig1: Western blot analysis on different cell lysates using anti-Hsp90 alpha Mouse mAb. Positive control:
  Lane 1: Hela
  Lane 2: 293T
  Lane 3: PC12
  Lane 4: NIH/3T3

   

  

  Fig2: Immunocytochemical staining of Hela cells using anti-Hsp90 alpha Mouse mAb.

   

  

  Fig3: Immunocytochemical staining of SW480 cells using anti-Hsp90 alpha Mouse mAb.

   

  

  Fig4: Immunocytochemical staining of Ags cells using anti-Hsp90 alpha Mouse mAb.

   

  

  Fig5: Immunocytochemical staining of NIH/3T3 cells using anti-Hsp90 alpha Mouse mAb.

   

  

  Fig6: Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-Hsp90 alpha Mouse mAb.

   

  

  Fig7: Immunohistochemical analysis of paraffin- embedded human gastric carcinoma tissue using anti-Hsp90 alpha Mouse mAb.

   

  

  Fig8: Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-Hsp90 alpha Mouse mAb.

   

  

  Fig9: Flow cytometric analysis of Hela cells with Hsp90 alpha antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the second

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